Yookamurra falls within the southern most extension of the semi-arid zone of South Australia. Temperatures vary greatly with very hot summers and cool winters. February is the hottest month of the year with an average daytime temperature of 32°C and July is generally the coldest month with an average daytime temperature of 17°C. The maximum temperature recorded at Yookamurra is 47°C and the overnight minimum -6°C. Average rainfall at the Sanctuary is 270 mm, the majority of which occurs between May and August.